ZHOU Fang-Liang, HE Lan, HE Dan, WANG Xian-Wen, SHI Hong-Jian, HE Ying-Chun, CAO De-Liang. Yi Qi Jie Du Decoction Inhibits Proliferation and Induces Apoptosis of Nasopharyngeal Carcinoma Stem Cells Through Mitochondrial Apoptosis Pathway[J]. Digital Chinese Medicine, 2019, 2(4): 219-226. DOI: 10.1016/j.dcmed.2020.01.003
Citation: ZHOU Fang-Liang, HE Lan, HE Dan, WANG Xian-Wen, SHI Hong-Jian, HE Ying-Chun, CAO De-Liang. Yi Qi Jie Du Decoction Inhibits Proliferation and Induces Apoptosis of Nasopharyngeal Carcinoma Stem Cells Through Mitochondrial Apoptosis Pathway[J]. Digital Chinese Medicine, 2019, 2(4): 219-226. DOI: 10.1016/j.dcmed.2020.01.003

Yi Qi Jie Du Decoction Inhibits Proliferation and Induces Apoptosis of Nasopharyngeal Carcinoma Stem Cells Through Mitochondrial Apoptosis Pathway

  • Objective To assess the effects of Yi Qi Jie Du Decoction (YQJDD) on nasopharyngeal carcinoma stem cells (NPC-SCs) and investigate the underlying mechanism.
    Methods NPC-SCs were collected in serum-free culture system and identified by the sphere formation assay. The effect of YQJDD on the proliferation of NPC-SCs was detected using the Cell Counting Kit-8 assay. The change of mitochondrial membrane potential (ΔΨm) in NPC-SCs following treatment with YQJDD was investigated by JC-1 staining. The levels of apoptosis-associated proteins were examined using western blot analysis.
    Results YQJDD significantly inhibited the proliferation of NPC-SCs in dose- and time-dependent manners. JC-1 staining revealed that YQJDD lowered the ΔΨm of NPC-SCs in a time-dependent manner. After treatment with YQJDD, the expression of cleaved caspase-3, -7 and -9, cleaved poly-ADP ribose polymerase, P21 and P53 were increased, while the expression of Survivin in NPC-SCs was decreased. However, the expression of cleaved caspase-8 remained nearly unchanged.
    Conclusions YQJDD inhibits the growth and induces apoptosis of NPC-SCs via an intrinsic apoptosis pathway.
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