大麻槿治疗尿石症和胆石症的临床前评价

SwathiSuresh; AnkulSingh S; ChitraVellapandian

doi:10.1016/j.dcmed.2023.07.008

大麻槿治疗尿石症和胆石症的临床前评价

Preclinical evaluation of Hibiscus cannabinus Linn. in the treatment of urolithiasis and cholelithiasis

摘要

摘要:
目的探究大麻槿叶对尿石症和胆石症的疗效。
方法评价大麻槿叶水提物对硫脲嘧啶和胆固醇胆酸饮食诱导的BALB/c小鼠胆石症和乙二醇诱导的Wistar大鼠尿石症的治疗作用。选取3个剂量的水提物( 40、80和160 mg/kg)对小鼠胆石症的药效进行评价；另外3个剂量的水提物( 400、800和1600 mg/kg)对大鼠尿石症的药效进行评价。在胆石症模型中测定胆汁胆固醇、胆汁磷脂、胆汁酸等生化指标；在尿石症模型中测定24小时尿量及肌酐、尿酸、蛋白质、尿素、草酸钙结晶、红细胞和脓尿等尿参数。
结果给予3个试验剂量的大麻槿叶水提物后，动物的胆道和泌尿系统参数差别均具有统计学意义( P < 0.05)。
结论大麻槿对高脂致石饮食导致的尿石症和胆石症具有明显疗效。

Abstract:
ObjectiveTo investigate the therapeutic effect of Hibiscus cannabinus Linn. (H. cannabinus) leaves on cholelithiasis and urolithiasis.
MethodsThe study evaluated the effect of aqueous leaf extract of H. cannabinus on thiouracil and cholesterol cholic acid diet induced cholelithiasis in BALB/c mice and ethylene glycol induced urolithiasis in Wistar rats. Three doses of aqueous extract (40, 80, and 160 mg/kg) were selected to evaluate the effectiveness in cholelithiasis in mice; another three doses of aqueous extract (400, 800, and 1600 mg/kg) were administered for evaluating the effect on urolithiasis in rats. Biochemical parameters such as biliary cholesterol, biliary phospholipid, and bile acid were determined in cholelithiasis model. Similarly, 24-hour urine output, urinary parameters such as creatinine, uric acid, protein, urea, presence of calcium oxalate crystals, red blood cells (RBCs), and pyuria were determined in urolithiasis model.
ResultsStatistically significant differences were noted in the biliary and urinary parameters after administrating three test doses of H. cannabinus aqueous extract (P < 0.05).
ConclusionH. cannabinus was found to be effective against high fat lithogenic diet urolithiasis and cholelithiasis.

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