基于UPLC-Q-TOF-MS技术鉴定卷丹百合不同部位中化学成分及其降血糖活性研究

张罗琴; 吴一振; 王洪; 向晟; 王冰卉; 谭晓文; 李中正

doi:10.1016/j.dcmed.2024.01.006

基于UPLC-Q-TOF-MS技术鉴定卷丹百合不同部位中化学成分及其降血糖活性研究

Identification of metabolites in different parts of Juandan Baihe (Lilium lancifolium) by UPLC-Q-TOF-MS and their hypoglycemic activities

摘要

摘要:
目的基于超高效液相色谱-四极杆飞行时间质谱（UPLC-Q-TOF-MS）技术鉴定卷丹百合不同部位提取物中的主要成分，并对其降血糖活性进行研究。
方法对百合中主要类型化合物的质谱裂解规律进行研究，基于质谱裂解规律结合质谱挖掘技术对卷丹百合不同部位提取物中的化学成分进行系统鉴定。并基于链脲佐菌素诱导（每天灌胃给药80 mg/kg，连续给药3天）的高血糖雄性小鼠模型无特定病原体(SPF)昆明小鼠，通过测量小鼠的血糖、日饮水量、日采食量及体重变化情况评价卷丹百合根、茎、球茎、叶和花提取物的降血糖活性。
结果本研究阐明了卷丹百合中王百合苷类、薯蓣皂苷类、咖啡酸类、黄酮苷类和绿原酸类化合物的质谱裂解规律，构建了百合植株中化合物的挖掘方法，从卷丹百合的根、茎、球茎、叶和花中共鉴定了58个化合物，包括绿原酸类化合物6个，王百合苷类化合物14个，咖啡酸类化合物13个，黄酮苷类化合物5个，薯蓣皂苷类化合物20个。其中30个化合物首次在百合中报道。卷丹百合根及球茎提取物表现出显著的降血糖活性，使高血糖模型小鼠血糖水平从最初的23.76 ± 1.21 mmol/L和24.29 ± 1.35 mmol/L下降到17.21 ± 1.23 mmol/L和18.78 ± 1.49 mmol/L （P < 0.05）。卷丹百合根及球茎提取物还能改善糖尿病引起的多饮（P < 0.01）、多食（P < 0.05）及体重减轻的症状。
结论本研究首次阐明卷丹百合根含有丰富的王百合苷类和薯蓣皂苷类化合物，并具有显著的降血糖活性，为百合植株的综合利用及降血糖药物的开发奠定基础。

Abstract:
Objective To identify the main components in the extracts of different parts of Juandan Baihe (Lilium lancifolium) by ultra-high performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) technology and investigate their hypoglycemic activities.
Methods The MS fragmentation pathways of the main types of compounds in Juandan Baihe (Lilium lancifolium) were studied, and the main components in the extracts were systematically identified using MS fragmentation pathways combined with MS mining technology. Based on the hyperglycemia male mouse model specific pathogen free (SPF)-grade Kunming mice induced by streptozotocin (intragastric administration of 80 mg/kg for 3 d), the hypoglycemic effects of extracts of Juandan Baihe (Lilium lancifolium) roots, stems, corms, leaves, and flowers were evaluated by measuring the changes of blood glucose, daily water consumption, daily food intake, and body weight.
Results The MS fragmentation pathways of regalosides, dioscins, phenylpropanoids, flavonoids, and chlorogenic acids in Juandan Baihe (Lilium lancifolium) were clarified, and a mining method for compounds in this plant was constructed. A total of 58 compounds, including 6 chlorogenic acids, 14 regalosides, 13 phenylpropanoids, 5 flavonoids, and 20 dioscins, were identified from the roots, stems, corms, leaves, and flowers of Juandan Baihe (Lilium lancifolium). Among them, 30 compounds were reported for the first time from this plant. The root and corm extracts demonstrated significant hypoglycemic activities by reducing blood glucose levels from 23.76 ± 1.21 and 24.29 ± 1.35 mmol/L to 17.21 ± 1.23 and 18.78 ± 1.49 mmol/L, respectively (P < 0.05). The roots and corms extracts could also attenuate the symptoms of polydipsia (P < 0.01), polyphagia (P < 0.05), and weight loss caused by diabetes.
Conclusion This study clarifies that the roots of Juandan Baihe (Lilium lancifolium) are rich in regalosides and dioscins for the first time, and have significant hypoglycemic activities, providing the foundation for the comprehensive utilization of this plant and the development of hypoglycemic drugs.

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