密蒙花颗粒对去势雄兔泪腺细胞凋亡因子Bax、Bcl-2、Fas和FasL的影响

Effect of Flos Buddlejae Granules on Apoptosis Factors Bax, Bcl-2, Fas and FasL in Lacrimal Gland Cells of Castrated Male Rabbits

    摘要
  • 摘要:
    目的 观察不同浓度密蒙花颗粒对去势雄兔泪腺细胞凋亡因子Bax、Bcl-2、Fas和FasL的影响，探讨密蒙花颗粒对去势雄兔干眼症模型的疗效。
    方法 将密蒙花原药材制备成颗粒。将30只健康成年新西兰长耳白兔（雄性），随机分为A：空白组；B：模型组；C：密蒙花颗粒组；D：安慰剂；E：睾酮组，每组6只。除A组外其余4组实验用兔行双侧睾丸及附睾切除术。除A、B组外，从术后第3天开始C组予密蒙花颗粒，按100 mg/kg灌胃，3次/日；D组予生理盐水灌胃；E组按0.5 mL/kg在大腿肌肉处注射丙酸睾酮注射液，每3天注射一次。各组实验用兔，分别于术前和术后第4周各进行一次Schirmer I试验(SIT)和泪膜破裂时间（BUT）测定。用药观察4周后采用空气栓塞法处死各组兔子，摘取双眼泪腺组织，采用免疫组化法对泪腺细胞凋亡因子Bax、Bcl-2、Fas和FasL的表达进行检测，并进行统计学分析。
    结果（1）SIT与BUT值比较：B、D组手术前后比较，P＜0.01，有明显统计学意义；其他各组手术前后比较，P＞0.05，无统计学意义；（2）术后各组凋亡因子Bax、Bcl-2、Fas和FasL比较：术后B、D组比较，P＞0.05，无统计学意义；B、D组与其他各组比较，P＜0.01，有明显统计学意义；A、C、E组间比较，P＞0.05，无统计学意义。
    结论 与雄激素比较，密蒙花颗粒抑制Bax、Fas和FasL的表达，上调Bcl-2的表达水平。

     

    Abstract:
    ObjectiveTo observe the effect of different concentration Flos Buddlejae (Mi Meng Hua, 密蒙花) granules on apoptosis factors of lacrimal gland cells of castrated male rabbits, and study the treatment effects of Flos Buddlejae (Mi Meng Hua, 密蒙花) granules in the dry eye model of castrated male rabbits.
    Methods Flos Buddlejae (Mi Meng Hua, 密蒙花) raw material was made into granules. Thirty healthy adult New Zealand rabbits were randomly divided into five groups, six rabbits in each group. Group A: blank group, Group B: model group, Group C: Flos Buddlejae (Mi Meng Hua, 密蒙花) granule group, Group D: placebo group, Group E: testosterone group. Except for group A, all rabbits underwent removal of bilateral testis and epididymis. Rabbits in group C were administered Flos Buddlejae (Mi Meng Hua, 密蒙花) granules (100 mg/kg), three times per day; rabbits in group D were administered normal saline, three times per day. Rabbits in group E were injected with testosterone propionate (0.5 mL/kg) in the thigh muscle, every 3 days. All rabbits were tested by Schirmer I test (SIT) and tear film break-up time (BUT) before operation and 4 weeks after operation. After 4 weeks, all rabbits were sacrificed by air embolism and clipping of the lacrimal gland. Apoptosis factors, including Bax, Bcl-2, Fas and FasL of lacrimal gland cells were characterized by immunohistochemistry, and resulting data were statistically analyzed.
    Results(1) Comparison of SIT and BUT before and after operation: There were statistically significant differences between groups B and D (P < 0.01), but not among other groups (P > 0.05). (2) Comparison of apoptosis factors Bax, Bcl-2 Fas and FasL: In a comparison of groups B and D, there was no statistically significant difference after operation (P > 0.05). In a comparison of the other groups, there were statistically significant differences (P < 0.01). In comparisons among A, C and E groups, there were no statistically significant differences (P > 0.05).
    Conclusion Compared with androgen, Flos Buddlejae (Mi Meng Hua, 密蒙花) granules caused similar but slightly weaker depression of Bax, Fas and FasL, and increased expression of Bcl-2.

     

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